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Poster Presentations
Day 3, June 24（Tue.）
Room P (Maesato East, Foyer, Ocean Wing)
- 3P-AM-08
Differentially expressed proteins in 4-cell stage porcine embryos derived from somatic cell nuclear transfer and parthenogenetic activation
(1NCHU Univ., 2ATRI, 3CMU)
oShu-Ping Chang1, Chi-Hong Wong2, Shu-Hui Peng2, Hsin-Yi Liao3, Pin-Chi Tang1, Ching-Fu Tu2, Chao-Jung Chen3, San-Yuan Huang1
The embryos derived from the somatic cell nuclear transfer (SCNT) technique encounter cloning deficiency and developmental problems. Developmental defects of SCNT embryos first appear at the time of zygotic genome activation (ZGA), which occurs in 4-cell stage in porcine, postulated that SCNT embryos have difficulties in ZGA. This study aimed to investigate the differential expression of proteins (DEPs) in 4-cell stage SCNT porcine embryos and parthenogenetically activated embryos. Embryonic proteins were extracted from approximately 100 embryos and subjected to label-free proteomic quantification with mass spectrometry. A total of 1402 protein groups were identified and quantified. Proteins with fold change > 2 or < 0.5 and significance > 13 were defined as DEPs. Consequently, 353 proteins showed downregulated and 78 upregulated in SCNT embryos. Biological process enrichment analysis showed that the DEPs were enriched in carboxylic acid metabolic process, generation of precursor metabolites and energy, and tRNA aminoacylation for protein translation. KEGG pathway enrichment revealed that carbon metabolism, proteosome, and protein processing in endoplasmic reticulum were involved. Additionally, ribosomal, proteasome and aerobic respiration related proteins showed strong interaction networks according to the STRING database. In conclusion, these DEPs may cause an imbalance in energy metabolism, proteasome function and translation, leading to developmental obstacles in SCNT embryos.