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Poster Presentations
Day 3, June 24（Tue.）
Room P (Maesato East, Foyer, Ocean Wing)
- 3P-AM-07
Development of In-situ High-pressure Electrospray Mass Spectrometry for Continuous Flow Hydrothermal Reactor
(1Univ. of Yamanashi/HDU, 2HDU, 3Univ. of Yamanashi)
oXiang Zhang1, Zhi Hua Ying2, Lee Chuin Chen3
Conventional ESI source has been used to monitor the chemical reactions in situ but the operating temperature is limited by the boiling point of the solution. Previously we showed that the high-pressure ESI source could handle subcritical water in addition to achieving electrical discharge-free electrospray for aqueous solution.
Here, we report our recent development of a high-temperature high-pressure ESI source that is coupled to a continuous flow hydrothermal reactor system (Fig.1). The system consists of a sealed ESI source that is filled with compressed air and a continuous flow hydrothermal reactor made of resistively heated stainless-steel capillary (SUS 304). A flow reducer with an inner diameter of 20 μm is introduced between the hydrothermal reactor capillary and the ESI source to increase the pressure in the reactor capillary, thereby allowing the solution to be heated up to the supercritical point. The outlet capillary of the ESI source can also be heated to perform post-ESI heating of the ions and charged droplets. The system was coupled to an orbital trap mass spectrometer.
The thermal-induced acid hydrolysis of ubiquitin and the effect of post-ESI pyrolysis were performed to obtain the fragmentation pattern under different temperatures and heating conditions. The in-situ monitoring of thermally induced oligomerization of alanine and glycine was also performed to determine the condition that promotes the formation of dipeptides.