| Timetable |
| Download Conference Program |
| Download All Abstracts |
| Zoom Access |
| Corporate Program |
Poster Presentations
Day 3, June 24（Tue.）
Room P (Maesato East, Foyer, Ocean Wing)
- 3P-AM-05
Biomarker Discovery of Cerebrospinal Fluids for the Infantile Epilepsy by the Lipidomic Profiling of Extracellular Vesicles
(1Teikyo Univ., 2Okayama Univ., 3Tohoku Univ.)
oArisa Ishii1, Tomoko Fukuuchi1, Noriko Yamaoka1, Tomoyuki Akiyama2, Daisuke Saigusa1,3
Epilepsy is a brain disorder that causes seizure symptoms. Although ectroencephalography (EEG) is the primarily diagnosis of epilepsy, the EEG of infants is unstable. Therefore, it has been required a novel biomarker for the prediction of epilepsy. The extracellular vesicles (EVs) are consisted with the lipid bilayers and involved with the cell signaling, and included in the cerebrospinal fluid (CSF). Therefore, we first extract the EVs from human CSF samples, and analyze their lipid species to identify biomarkers that may contribute to the clinical diagnosis.
CSF (200 μL) was prepared from epileptic and non-epileptic patients (n = 15, each). EVs were extracted by MacCaptureTM exosome isolation kit. The lipid species were extracted from the isolated EVs solution followed by the Bligh & Dyer method, and the sample was subject to UHPLC-FTMS. The lipid species were identified with the LipidSearch software.
In total 3,870 lipid species were annotated in EVs derived from CSF. Some of the lipid species were significantly higher or lower in epileptic compared to the non-epileptic samples. This study suggests that EVs in CSF of patients with epilepsy may originate from changes in lipid rafts of neuroaxons, and they will be the candidates for the prediction of epilepsy.