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Oral Sessions
Day 3, June 24（Tue.） 16:30-16:45
Room C (Top of Yaima)
- 3C-O3-1630
Development and Application of Unified-HILIC/AEX/MS for Comprehensive and Practical Metabolomics
(1Niigata Univ., 2Kyushu Univ./MiB)
oKohta Nakatani1,2, Yoshihiro Izumi2, Masatomo Takahashi2, Takeshi Bamba2
Metabolomics is a comprehensive study of metabolites in biological samples, with liquid chromatography-mass spectrometry (LC/MS) being a widely used analytical technique. However, conventional methods face challenges in comprehensiveness, stationary phase durability, and retention time stability. There is a strong demand for a versatile and accessible first-choice method.
To address these issues, we developed unified-HILIC/AEX, a novel separation mode integrating hydrophilic interaction liquid chromatography (HILIC) and anion-exchange chromatography (AEX). This method employs a mixed amine-modified polymer column and an alkaline mobile phase with a salt concentration gradient. In the first stage, anionic metabolites are adsorbed onto the positively charged stationary phase while others are separated by HILIC mode. In the second stage, the retained anions are separated by AEX mode. When coupled with mass spectrometry (unified-HILIC/AEX/MS), this method achieved 1.6 times more metabolite peaks than conventional approaches, demonstrating superior comprehensiveness. To establish it as a standard method, further evaluations of durability, stability, and mass spectrometer compatibility are required.
This report presents retention time variations, mass spectrometry applications, and biological sample analyses, including cultured cells, media, organs, blood, and immune cells. The unified-HILIC/AEX/MS method is a powerful, user-friendly, and practical approach, highlighting its potential as a next-generation analytical technique.