| Timetable |
| Download Conference Program |
| Download All Abstracts |
| Zoom Access |
| Corporate Program |
Oral Sessions
Day 3, June 24（Tue.） 16:15-16:30
Room C (Top of Yaima)
- 3C-O3-1615
A High-Sensitivity Derivatization Strategy for Enhanced Hydroxyl Metabolite Detection in LC-MS and DESI-MS
(1National Yang Ming Chiao Tung University, 2National Taiwan University Hospital, 3National Taiwan University)
Yen-Chu Lin1, Guan-Yuan Chen2,3, oHsiao-Wei Liao1
Chemical derivatization plays a crucial role in LC-MS metabolomics by improving ionization efficiency, enabling enhanced detection of metabolites in complex biological matrices. 2-(4-Boronobenzyl) isoquinolin-2-ium bromide (BBII), originally developed as a glucose-labeling reagent for matrix-assisted laser desorption/ionization MS, has demonstrated significant potential for hydroxyl metabolite detection due to its permanently charged quaternary ammonium group, which improves ionization. We previously applied BBII in post-column derivatization (PCD) to enhance hydroxyl metabolite sensitivity in LC-MS, enabling the detection of previously unobservable hydroxyl metabolites such as glucose, ribose, and long-chain alcohols, with sensitivity enhancements ranging from 1.1- to 42.9-fold.
Building on this foundation, we further extend BBII derivatization to desorption electrospray ionization (DESI) for hydroxyl metabolite detection. By incorporating BBII into the electrospray solvent, hydroxyl metabolite reference standards were successfully derivatized in situ and detected using BBII-assisted DESI. This real-time derivatization strategy not only facilitates rapid hydroxyl metabolite detection but also enables high-sensitivity metabolite imaging.
BBII-assisted DESI offers a novel and effective approach for hydroxyl metabolite detection, broadening its applications in mass spectrometry imaging. This strategy expands the utility of ambient ionization techniques, allowing direct analysis of biological samples with minimal preparation.