The 10th Asia-Oceania Mass Spectrometry Conference (AOMSC2025) - organized by the Mass Spectrometry Society of Japan

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Oral Sessions

Day 3, June 24(Tue.) 14:10-14:25

Room C (Top of Yaima)

  • 3C-O2-1410
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Localization Analysis of Metabolites in Living Cells by Live Single-cell Mass Spectrometry

(1Meijo Univ., 2Univ. Shizuoka, 3Yokogawa Electric Corp., 4ITO EN)
oHajime Mizuno1, Aogu Furusho2, Takuma Yanagisawa1,2, Eiji Sugiyama1, Yuta Terui3, Masafumi Iharada3, Hironori Takai3, Susumu Imanishi1, Kenichiro Todoroki2, Iwao Sakane4

There are increasing demands to study the molecular and cellular behavior of a single cell precisely because the current knowledge in life science was obtained using a lot of cells with little information on the localization or environment of cells. We developed the “Live Single-cell Mass Spectrometry" method which allows direct analysis of xenobiotics and endogenous compounds in a living cell. This method is comprised of two steps; 1. cellular contents are captured from a living cell using a metal coated glass microcapillary (i.d. 3-10 μm) under microscopic observation, and 2. the capillaries are directly subjected to static nano-electrospray ionization of the sampled cellular contents such as cytosol or organelles for mass spectrometry. We are currently trying to sample intracellular organelles more accurately using an automatic sampling device (SS2000, Yokogawa Electric Corporation). This system is capable of sampling either a targeted whole living cell or an organelle in a cell automatically based on a captured 3D confocal bioimage. In this study, we will report on the localization analysis of drugs and endogenous phospholipids in single drug-treated cells.