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Oral Sessions
Day 3, June 24（Tue.） 11:25-11:40
Room B (Maesato Center)
- 3B-O1-1125
Developments of laser desorption Post-photoionization Mass Spectrometry Imaging Techniques
(Univ. Sci. Tech. China)
oYang Pan
Laser-based high-resolution mass spectrometry imaging at ambient conditions has promising applications in life science. However, the ion yield during laser desorption/ablation is poor. Recently, great attention has been paid to the post-photoionization induced by a krypton discharge lamp, which emits high-energetic vacuum ultraviolet (VUV) light (10.0 and 10.6 eV)1-3. Here, atmospheric pressure laser desorption combined with a compact postphotoionization (AP-LDI/PI) assembly with a krypton discharge lamp was developed for the untargeted imaging of various biomolecules. The spatial distributions of numerous lipid classes, fatty acids, neurotransmitters, and amino acids in subregions of mouse cerebellum tissue were obtained. Compared with single laser ablation, the sensitivities for most analytes were increased by 1 to 3 orders of magnitude by dopant-assisted postphotoionization. After careful optimization, a spatial resolution of 4 μm could be achieved for the metabolites in mouse hippocampus tissue. Finally, the melanoma tissue slices were analyzed using AP-LDI/PI MSI, which revealed the metabolic heterogeneity of the melanoma microenvironment and exhibited the phenomenon of abnormal proliferation and invasion trends of tumor cells.