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Oral Sessions
Day 3, June 24（Tue.） 16:45-17:00
Room A (Maesato West)
- 3A-O3-1645
Synthesis of Core-Shell Mesoporous Silica-TiO₂ Nanocomposite Functionalized with Boronic Acid for Selective and Efficient Glycopeptide Enrichment
(CNU)
oMohamed Gab-Allah, Jeongkwon Kim
Protein glycosylation plays a crucial role in various physiological processes and is strongly associated with disease progression, particularly in cancer. However, effectively capturing N-glycopeptides with high specificity and recovery from complex biological samples remains a major challenge. In this study, we present a novel enrichment strategy utilizing a boronic acid-functionalized core-shell mesoporous silica–TiO₂ nanocomposite (referred to as CSMS@TiO₂–GLYMO–APB) for the selective isolation of intact glycopeptides. The synthesized nanomaterial offers several key advantages, including the hydrophilic properties of silica, a high BET surface area of 122.8 m²/g, a well-developed porous structure with an 8.5 nm pore size, and boronic acid functionality. These features collectively enhance the selective and efficient enrichment of glycopeptides from biological samples. The glycopeptide enrichment performance was assessed by capturing IgG N-glycopeptides, leading to the identification of 35 glycopeptides by MALDI-TOF MS. Additionally, 215 glycopeptides corresponding to 91 glycoproteins were successfully enriched from only 2 µL of serum, demonstrating the effectiveness and potential of this approach for intact glycopeptide analysis.