The 10th Asia-Oceania Mass Spectrometry Conference (AOMSC2025) - organized by the Mass Spectrometry Society of Japan

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Day 3, June 24(Tue.) 16:15-16:30

Room A (Maesato West)

  • 3A-O3-1615
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Comparison of the components of the Liocheles australasiae scorpion venom between different growth stages or colors

(Kyoto Univ.)
Kentaro Kojima, Ryo Shimase, Yoshiaki Nakagawa, oMasahiro Miyashita

Scorpions use venom to capture prey and to protect from predators. The major components of scorpion venom are peptides, some of which shows insecticidal activity. Scorpions use venom even when they are juveniles, but changes in venom components among the growth stages remain unclear. Scorpions are also known to secrete two types of venom: transparent and opaque. However, its biological significance and differences in components in each venom have been poorly understood. In this study, we compared the amounts of five insecticidal peptides in the venom of Liocheles australasiae between juveniles and adults and between transparent and opaque forms.
First, the venom from second-instar juveniles and adults was analyzed by LC/MS. As a result, there were differences in the abundance of some insecticidal peptides between juveniles and adults. This suggested that this scorpion uses venom with different compositions depending on their growth stages. Next, the composition of transparent and opaque venom was compared. The results showed that the amount of all insecticidal peptides was low in the transparent venom, suggesting that this scorpion use two different sets of the venom components depending on the situation.