Timetable |
Download Conference Program |
Download All Abstracts |
Zoom Access |
Corporate Program |
Oral Sessions
Day 3, June 24(Tue.) 15:45-16:15
Room A (Maesato West)
- 3A-O3-1545
Mass Spectrometry in the de novo Sequencing of Bioactive Non-tryptic Amphibian Peptides
(MSU-BIT Shenzhen Univ.)
oAlbert Lebedev, Tatiana Samgina
Amphibians live on Earth for hundreds of millions of years. Their dorsal glands produce biologically active peptides, which successfully fight microorganisms and even predators. Skin secretion of amphibians contains wide spectrum antibiotic and neuropeptides active at the levels of 10-9 М. Antimicrobial peptides are very perspective pharmaceuticals of future generations.
Skin secretions of various frog species were obtained by mild electrostimulation. Mass spectrometry was applied as a unique and the most powerful proteomic tool. Over 200 peptides were sequenced in the present study. The developed de novo sequencing algorithm enables achieving complete sequence coverage of frog peptides. It resolves problems of S-S bonds and cyclization of short peptides. EThcD and ExD tools efficiently and reliably differentiate isomeric Leu/Ile.
Peptidome representation with 2D-maps based on MS parameters is a convenient method in taxonomy to distinguish frogs of closely related species and even populations. It involves changes in the sequences of similar peptides due to diversity of natural habitat. Although various peptides are encoded in the DNA, environmental situation triggers formation of only particular ones, depending on the climatic conditions. As a result, populations of the same frog species may be distinguished using the information on their skin peptides' sequences.