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Oral Sessions
Day 3, June 24（Tue.） 14:10-14:25
Room A (Maesato West)
- 3A-O2-1410
Comprehensive Glycomic Profiling of Immunoglobulin G, A, and M in Tuberculosis: Insights into Active and Latent Infection in the Elderly
(1Taipei Medical University, Taiwan, 2Wan-Fang Hospital, Taiwan)
Yun-Jung Yang1, Chih-Hsin Lee2, oI-Lin Tsai1
Tuberculosis (TB), caused by Mycobacterium tuberculosis (Mtb), remains a major global health challenge, particularly among the elderly, where distinguishing active TB (ATB) from latent TB infection (LTBI) is critical for disease management. While IgG glycosylation has been studied in TB, alterations in IgA and IgM glycosylation remain poorly understood. This study investigated glycosylation patterns of IgG, IgA, and IgM in 59 participants (22 ATB, 17 LTBI, and 20 controls) using a dual-enzyme workflow, affinity purification, and LC-MS/MS analysis.
ATB patients exhibited lower galactosylation and higher fucosylation of IgG and IgM, along with novel glycosylation shifts in IgA at N144/131. These glycosylation changes suggest an enhanced inflammatory response in ATB. Receiver operating characteristic (ROC) analysis yielded an area under the curve (AUC) of 0.808 when integrating glycosylation traits from all three immunoglobulins, demonstrating improved diagnostic accuracy compared to IgG alone. These findings provide new insights into TB-associated immune glycomic alterations and highlight IgA and IgM glycosylation as promising biomarkers for distinguishing ATB from LTBI. Understanding these glycosylation changes could lead to more accurate diagnostic strategies, particularly for elderly individuals at higher risk of TB reactivation and progression.