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Poster Presentations
Day 2, June 23（Mon.）
Room P (Maesato East, Foyer, Ocean Wing)
- 2P-PM-13
Proteomic Insights into Plant-PGPR Interactions: Elucidating Key Mechanisms During Commensalism
(Academia Sinica)
oKai-Ting Fan, Ching-Huang Yu, Yet-Ran Chen
Plants recruit plant growth-promoting rhizobacteria (PGPR) to enhance growth, nutrient uptake, and stress tolerance while inducing systemic resistance. However, inconsistent PGPR performance between laboratory and field conditions remains a challenge due to limited understanding of plant-PGPR interactions. To address this, we employed quantitative proteomics using a data-independent acquisition approach to investigate dynamic regulatory events in Arabidopsis roots and shoots during Bacillus subtilis colonization. Using a hydroponic system optimized for stable B. subtilis root colonization, we identified three key stages, representing early, late colonization, and stable commensalism. In early colonization, root proteins involved in secondary metabolite biosynthesis, JA biosynthesis and signaling were upregulated, while PR1 upregulation in shoots suggested systemic resistance via SA-PR1 signaling. During late colonization, lignin biosynthesis proteins were upregulated in roots for cell wall fortifying, while resource reallocation from defense to growth occurred in shoots. During stable commensalism, carotenoid biosynthesis downregulation in roots suggested altered root architecture, while upregulated energy metabolism and developmental proteins in shoots, along with reduced ABA biosynthesis and immune receptor signaling, indicated the overall enhanced growth and PGPR compatibility. These findings reveal stage-specific proteomics dynamics in plants during commensalism, providing insights for optimizing PGPR applications in agriculture.