The 10th Asia-Oceania Mass Spectrometry Conference (AOMSC2025) - organized by the Mass Spectrometry Society of Japan

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Poster Presentations

Day 2, June 23(Mon.) 

Room P (Maesato East, Foyer, Ocean Wing)

High-Resolution Imaging and Rapid Detection of Ustalic Acid in Tricholoma kakishimeji Using MALDI-IMS and PESI-MS/MS

(1Gifu Univ. Med. Sci., 2Nagoya Univ., 3Fukushima Univ., 4Shinshu Univ., 5Gifu Pref. Inst. Health Env. Sci.)
oTetsuro Ito1, Shintaro Aritaki2, Shu Taira3, Wataru Aoki4, Mimori Tsuji4, Hiroyuki Nagai5, Masashi Fukaya1, Kaori Ryu1, Katsuhiro Shiratake2, Akiyoshi Yamada4

In previous research, we performed molecular phylogenetic analysis and morphological observations on Japanese Tricholoma species and two European specimens, identifying four undescribed species, including T. kakishimeji (TK), and the known species T. stans. We determined that TK is the only species producing ustalic acid (UA), a primary toxin, as detected by liquid chromatography–mass spectrometry (LC–MS).
This research examines the localization and detection of UA in TK using advanced mass spectrometry techniques. Imaging mass spectrometry (IMS) with matrix-assisted laser desorption/ionization (MALDI) revealed that UA is primarily located on the surface of the fruiting body, with higher concentrations in younger specimens. In addition, a rapid detection method employing probe electrospray ionization tandem mass spectrometry (PESI-MS/MS) was developed, demonstrating high sensitivity and specificity. This method enabled UA detection within 0.5 minutes and provided insights into its temporal production across various growth stages. The rapid detection of UA facilitates species identification, potentially allowing on-demand inspection without the delay associated with classical molecular phylogenetic, morphological, and LC–MS-aided chemical analysis. These findings advance the identification of toxic secondary metabolites in mushrooms and contribute to public health measures and food poisoning responses.