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Poster Presentations
Day 1, June 22（Sun.）
Room P (Maesato East, Foyer, Ocean Wing)
- 1P-PM-24
Increasing the Flexibility of Electrospray Ionization Mass Spectrometry by Introduction of Multiple Modifier Vapors
(NTHU CHEM)
oYing-Rong Hwang, Decibel Elpa, Pawel Urban
One of the ways to improve performance of electrospray ionization mass spectrometry (ESI-MS) is to introduce additives to the sample solution. Alternatively, such additives can be introduced in the gaseous form directly to the electrospray plume. Normally, only one additive can be introduced at a time. Here, we present a semi-automated system that enables dynamic switching among several gas-phase additives, which can be alternately introduced to the ion source compartment. These additives include vapors of acids and solvents. We evaluated two vapor delivery methods into the electrospray: sequential and gradient introduction. Due to the varying physicochemical properties of each analyte, predicting the optimal gas-phase additive is challenging. In sequential mode, we found that different gaseous additives enhance small molecule signals to varying degrees, making this approach useful for rapid additive screening. In gradient mode, we dynamically adjust the concentration of acid or solvent vapors by controlling the ratio of nitrogen gas to additive vapors. This allows us to observe changes in protein charge states and signal intensity during analysis. These results demonstrate the potential of our system for improving ionization efficiency and expanding the analytical capabilities of ESI-MS.