演題概要

3P-39

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Comprehensive Distribution Analysis of Cyclosporin A in Mice Tissues Using Four Mass Spectrometry Imaging Platforms

(¹NSU, Bangladesh・ ²HUSM, Japan)
^oIslam, Ariful¹・ Rahman, Md.²・ Afroz, Mst.²・ Mamun, Md.²・ Sakamoto, Takumi²・ Sato, Tomohito²・ Takahashi, Yutaka²・ Kahyo, Tomoaki²・ Setou, Mitsutoshi²

Comprehensive information of administered drugs in biological tissues is of paramount importance to understand their pharmacokinetics. Mass spectrometry imaging (MSI) that offers label-free visualization of drugs and their metabolites in the tissue sections. Recently, researchers are trying to develop peptide drugs for different diseases. However, it is still challenging to understand the pharmacokinetics of these drugs due to the lacking of an analytical technique to visualize them and their metabolites.
In this study, we tried to developed an analytical method for pharmacokinetic analysis of cyclic peptide drugs; Cyclosporin A (CsA) applying four different MSI tools: atmospheric pressure matrix-assisted laser desorption ionization (AP-MALDI)-MSI (iMScope QT, Shimadzu, Japan), Solarix XR 7T FT-ICR (Bruker Daltonics, Germany), desorption electrospray ionization (DESI)-MSI (Xevo G2-XS Q-TOF, Waters, USA), and DESI-TQ (Xevo TQ-Xs, Waters, USA). For that study, we used C57BL/6 mice injected with cyclosporin A. In this study, iMScope QT showed better detection capability for standard CsA compared to other three MSI tools. We also explored region specific accumulation of CsA and its metabolites in mice kidneys, jejunum, colon, and spleen using iMScope QT at higher spatial resolution (10 μm). Finding of our study may be helpful for the better understanding of the pharmacokinetics of CsA.