演題概要

3P-34

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電子励起乖離（EAD）を活用した乳製品中の機能性候補成分の構造解析

(¹明治・ ²京女大)
^o中山魁¹・ 松尾道憲²

We conducted an investigation to identify potential components in mold-fermented cheese (camembert cheese) that possess the capacity to enhance the expression of ABCA1, an ATP-binding cassette transporter associated with the elimination of cholesterol from peripheral tissues. Through the implementation of untargeted metabolomics approach using HRMS, a potential compound with the chemical formula C₁₆H₃₂O₃ was targeted. This compound was determined to be palmitic acid with a hydroxyl group by using CID-MS/MS spectra. However, it was ascertained that this compound differs from 2-hydroxy (α-position), 3-hydroxy (β-position), and 16-hydroxy (γ-position) palmitic acid. By utilizing electron activated dissociation (EAD), it was deduced that the compound in question was hydroxylated at the 10th position. Consequently, upon obtaining a synthetic standard reagent of 10-hydroxypalmitic acid and subjecting it to analysis, a concordance in retention time and MS/MS spectrum with the peak detected in camembert cheese was observed. These results confirmed that the candidate compound was 10-hydroxypalmitic acid. Furthermore, the ABCA1 expression enhancing effect was validated in the 10-hydroxypalmitic acid standard reagent, thereby confirming 10-hydroxypalmitic acid as one of the functional compounds that exhibits the ability to promote ABCA1 expression in camembert cheese.