演題概要

3P-31（3A-O1-1030）

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Exploring Proteomic Alterations Induced by the Infusion of Young Plasma during the Recovery from Traumatic Brain Injury in Mice

(¹CGU・ ²CGUBS・ ³CGUMMRC)
^oChen, Wen-Chen¹・ Chang, Wei-Che¹・ Chen, Hsiao-Wei³・ Huang, Guo-Jen²・ Chen, Yi-Ting²

Traumatic brain injury (TBI) induces substantial neuronal and systemic inflammation. Prior research has shown that young plasma could mitigate inflammation in mice with Alzheimer's disease. Consequently, we hypothesize that healthy mouse plasma has the potential to improve TBI pathologies.
In this study, TBI mice were injected with plasma from healthy mice. We discovered that the healthy plasma transfusion improved the motor and sensory abilities of TBI mice. We utilized Tandem Mass Tag (TMT)-based proteomics platform and nano-LC/MS-MS to explore the profiling differences in brain tissue proteome between TBI mice with plasma/saline transfusion.
Among the identified 8,656 proteins from the cortex tissue, 626 and 435 were discovered as increased or decreased proteins, respectively, based on their fold change (Mean± SD) as the cut-off values. Pathways analysis revealed that the most significant pathways were the Ferroptosis, NAD, and CLEAR signaling pathways. Ferroptosis, a pathway previously established to be associated with TBI, exhibited lower activity after plasma transfusion. Other upregulated pathways were associated with cell proliferation and apoptosis reduction.
Given these findings, young mouse plasma may influence these pathways, potentially alleviating TBI's pathological symptoms. In the future, we will analyze the plasma to identify TBI-related differential proteins for potential clinical applications.