ポスター発表
第3日 6月12日（水） P会場（多目的ホール・大会議室101＋102）P1会場（多目的ホール）・P2会場（会議室101＋102）
- 3P-30
糖タンパク質の部位特異的グライコフォーム解析に資するソフトウェア開発と応用
(1産総研細胞分子・ 2明薬大薬教セ・ 3名大糖鎖コア)
o岡谷千晶1・ 富岡あづさ1・ 富永大介1,2・ 坂上弘明1・ 久野敦1・ 梶裕之1,3
For effective site-specific glycoform analysis of glycoproteins, we have developed a Glyco-RIDGE method. Here, we describe a novel web tool named GRable for semi-automated Glyco-RIDGE analysis. This tool allows site-specific glycoform analysis of sialic acid-containing intact glycopeptides in a high-throughput manner. We also present a large-scale Glyco-RIDGE analysis of six mouse tissues, in which over 10,000 site-specific N-glycoforms were identified from 800 glycoproteins. Thus, this methodology can be applied to both in-depth analyses of target glycoproteins and large-scale analyses of crude glycoprotein samples, and GRable-assisted glycoproteomics allows for investigation of disease-related glycosylation alterations and thus can facilitate the accelerated development of glycomedicine. This web tool is freely available on the GlyCosmos Portal.