ポスター発表
第3日 6月12日（水） P会場（多目的ホール・大会議室101＋102）P1会場（多目的ホール）・P2会場（会議室101＋102）
- 3P-20
MALDI-DIT-MSを用いたアンチセンスモルフォリノ核酸の配列解析法
(島津)
o福山裕子・ 関谷禎規・ 岩本慎一・ 田中耕一
In recent years, the practical application of oligonucleotide therapeutics has rapidly progressed, and a simpler and more rapid analytical method is desired. We have previously reported antisense single-stranded DNA/RNA analysis and siRNA analysis using matrix-assisted laser-desorption/ionization digital-ion-trap mass spectrometer (MALDI-DIT-MS) (MSSJ, 2022, 3P-14; MSSJ, 2023, 2P-44). In this study, we report the antisense morpholino oligonucleotides analysis using MALDI-DIT-MS. Morpholino oligonucleotides are known to be difficult to fragment using collision-induced dissociation (CID). Last year, sequencing of morpholino oligonucleotides using electron-capture dissociation (ECD) combined with LC-MS was reported (Anal. Chem. 2023, 95, 16352-16358). Herein, we introduce morpholino oligonucleotides sequencing using MALDI-DIT-MS, which enables simpler and faster sequencing.