ポスター発表
第3日 6月12日（水） P会場（多目的ホール・大会議室101＋102）P1会場（多目的ホール）・P2会場（会議室101＋102）
- 3P-15
LC-MS用いたアルド-ケトレダクターゼ阻害薬の阻害効果評価方法の検討
(1武庫女大薬・ 2神戸薬大・ 3大阪産業技術研)
福田千紘1・ o堀山志朱代1・ 原史子1・ 髙田慎也1・ 萩森政頼1・ 佐野支帆子2・ 佐藤博文3・ 靜間基博3
The human aldo-keto reductase (AKR) superfamily plays an important role in a variety of biological functions. However, it is also associated with diseases such as diabetic neuropathy and breast cancer, so that these AKRs are a therapeutic target enzyme for these diseases. Unfortunately, due to the high homology of the AKR family, inhibiting only the target enzyme is not possible, resulting in side effects. In this study, we focused on AKR1B1 and AKR1B10, enzymes known to be involved in diseases such as diabetic neuropathy and breast cancer, and glutathione-conjugated carbonyl compounds (GSH-CA) were synthesized with substrate specificity for these enzymes. The study evaluates the inhibitory effect of AKR1B1 or AKR1B10 selective inhibitors on the enzyme activity using this substrate by LC/MS method.