演題概要

3D-O2-1451

PDF

ケミカルプローブ-GC/MSを用いた生体内脂肪酸β酸化の非侵襲的計測

(¹京大院医・ ²九大院薬)
^o松岡悠太¹・ 内之宮祥平²・ 王子田彰夫²・ 杉浦悠毅¹

Fatty acid oxidation (FAO) is a metabolic process that breaks down fatty acids into acetyl-CoA. It is crucial for energy production and contributes to maintaining homeostasis by adapting to various exogenous and endogenous stresses. To accurately understand the FAO response and regulation within living organisms, non-invasive and real-time measurement techniques for FAO are essential.
As a non-invasive method and indicator of energy metabolism, the respiratory quotient, calculated from the ratio of CO2 produced to O2 consumed by an individual, is often used. However, distinguishing the contribution of each energy production reaction and monitoring them using this indicator has been challenging.
In this study, we developed a non-invasive measurement method for in vivo FAO using volatile chemical probe and GC/MS. This probe releases volatile reporter molecules upon undergoing FAO in the body. Subsequently, these reporter molecules are emitted as biogas and detected by TD-GC/MS system, allowing for the non-invasive analysis of FAO within the body (Figure 1).
Upon adding the probes to HepG2 cells, the amount of reporter molecules in the cellular gas phase increased in an FAO-dependent manner. Furthermore, upon administering the probes to mice and analyzing the amount of reporter molecules released as biogas over time, an increase in the released amount was observed. Currently, we are verifying the applicability of this technique for monitoring in vivo FAO responses to endogenous stress. We will present these findings in this presentation.