演題概要

3D-O2-1415

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ヒト組織のハイブリッド・イメージング：脳組織の分子原子アトラスの創生

(¹獨協医大・ ²東大院理・ ³東薬大・ ⁴量研)
^o小川覚之¹・ 田中栄成²・ 名和大輝²・ 梅村知也³・ 武田志乃⁴・ 平田岳史²

In our body, various molecules constitute tissues and organs, and work efficiently to fulfill their physiological function in a coordinated and dynamic manner. When the molecular coordination breaks down for some reason, its pathological modulation appears as a disease. Therefore, it is important to obtain as much information as possible about the quality and localization of all molecules and atoms on the tissue and to analyze them precisely in an integrated manner. In this presentation, we introduce the integrated analyses of the same brain tissue by applying theoretically different imaging analyses, combining the conventional histological analyses with MALDI (matrix-assisted laser desorption/ionization)-IMS (imaging mass spectrometry), SALDI (surface-assisted laser desorption/ionization)-IMS, Nano-PALDI (Particle Assisted Laser Desorption/Ionization)-IMS, LA (laser ablation)-ICP-MS, PIXE (particle induced X-ray emission), and HE-SR-XRF (high-energy X-ray fluorescence spectrometry) imaging. Our studies show the integrated molecular imaging analyses is a promising approach that can deepen our knowledge about the localization of biological molecules in the central nervous system and disease brains during pathogenesis.