演題概要

3B-O2-1415

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Data-independent acquisitionリピドミクスを用いたグリセロ脂質代謝経路の¹³C代謝フラックス解析法の開発

(¹阪大院情報・ ²阪大島津協働研・ ³阪大先導研・ ⁴島津)
^o岡橋伸幸^1,2,3・ 山田侑季¹・ 原大樹¹・ 遠山敦彦⁴・ 飯田順子^2,4・ 松田史生^1,2,3

¹³C-metabolic flux analysis of lipid metabolism was developed using data-independent acquisition (DIA)-based lipidomics. Fragment ions corresponding to glycerol backbones and polar heads was investigated by LC-QTOF/MS. ¹³C-labeling data of the fragment ions of glycerolipids and phospholipids in budding yeast was analyzed. Flux distribution of a glycerolipid metabolic pathway was successfully estimated by the time-series ¹³C-labeling data.