若手研究者セッション
第3日 6月12日（水） 9:15～9:30 B会場（中ホール200）
- 3B-O1-0915（2P-31）
HILIC/MS/MSによるプロテオミクス分離基盤の構築
(1京大院薬・ 2医薬基盤健栄研)
o金尾英佑1,2・ 赤松幸真1・ 足立淳1,2・ 石濱泰1,2
Hydrophilic Interaction Chromatography (HILIC) coupled with liquid chromatography/tandem mass spectrometry (LC/MS/MS) is a promising technique for bottom-up proteomics. HILIC utilizes a high organic content solvent for the mobile phase, improving the MS sensitivity and throughput. Despite the potential, challenges in peptide separation robustness hinder practical application. To address this, a Two-step dissolution method was developed, optimizing peptide solubility with 25% ACN followed by adjusting to a final 95% ACN concentration. This approach significantly enhanced robustness, leading to the identification of over 1,200 proteins—20% more than conventional methods. The Two-step dissolution method demonstrated high reproducibility, suggesting its potential to improve the robustness of HILIC/MS/MS-based bottom-up proteomics.