演題概要

2P-35

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Quantitative Peptidomics Study for the Identification of a PAMP-Induced Phytocytokine that Regulate Plant Systemic Acquire Resistance

(¹ABRC・ ²IOB)
^oHung, Sheng-Chi^1,2・ Chen, Yet-Ran^1,2

To survive from unfavourable environments, plants have developed complex signalling mechanisms for adapting the diverse stress responses. Peptide hormones have been found to be the major players in activating and fine-tuning these signaling mechanisms. However, this class of biomolecules in plants are in general expressed dynamically and in sub-nM range, which brings a great challenge to study how peptide hormones are produced and regulated in plants. Here, a more efficient approach, based on data-independent acquisition (DIA), has been developed to study peptide hormone regulation in plant under various environmental stresses. Our investigations have unveiled a novel peptide induced by Pathogen Associated Molecular Patterns (PAMP) flagellin peptide flg22. This 12-amino acid peptide originates from the C-terminal region of its precursor protein and this peptide is functional in eliciting systemic acquired resistance (SAR) against pathogen Pseudomonas syringae pv. tomato DC3000 infections. In summary, our peptidomic approach has successfully identified and characterized a novel PAMP-induced peptide capable of instigating SAR. This approach can further applied to identify the novel functional peptide hormone in other stresses or different physiological changes in plants. With the more comprehensive understanding of the innate signaling in plants, we can develop the precision farming for the future agriculture.