ポスター発表
第2日 6月11日（火） P会場（多目的ホール・大会議室101＋102）P1会場（多目的ホール）・P2会場（会議室101＋102）
- 2P-34
涙液プロテオミクスにおけるDIA法の有効性評価
(1メニコン・ 2名大ITbM)
o吉満円香1・ 長屋大地1・ 加納圭子2・ 三城恵美2・ 角出泰造1
We have been analyzing changes in the protein of tear fluid using data dependent acquisition (DDA) proteomics to investigate the effect of contact lens wear on ocular surface. In this study, the efficacy of data independent acquisition (DIA) proteomics for tear fluid was evaluated. The tear samples collected from four subjects were analyzed using both DDA and DIA methods. The number of proteins identified using DIA method was about 3.7 times higher than DDA method (p < 0.01). Over 1,000 new proteins not detected by DDA method were detected by DIA method, including kallikrein and angiotensin-converting enzyme, which have been reported as biomarkers for glaucoma. Taken together, these results suggest that DIA method is effective for search of new biomarkers in tear fluid.