ポスター発表
第2日 6月11日（火） P会場（多目的ホール・大会議室101＋102）P1会場（多目的ホール）・P2会場（会議室101＋102）
- 2P-32
4D-LC/MS: オンライン酵素消化によるmAbペプチドマッピングの自動化
(アジレント)
o林明生・ 内藤厚子
Protein biopharmaceuticals have emerged as important therapeutics for the treatment of various diseases including cancer, cardiovascular diseases, infection, inflammatory, and autoimmune disorders. Together with a huge therapeutic potential, these molecules come with an enormous, analytically demanding structural complexity. A key technology to study cha0rge variants that might arise from PTMs such as asparagine deamidation, -C-term lysine truncation, N-term cyclization (pyro-Glu formation). CEX buffers are typically composed of nonvolatile constituents, making these methods incompatible with MS. We show that an automated online 4D-LC/MS integrating CEX (1D), peak collection, 2D desalting and reduction, 3D Lys-C digestion, and 4D C18 LC/MS-based peptide mapping can be executed in 120 min a fraction for the in-depth characterization of mAb charge variants.