ポスター発表
第2日 6月11日（火） P会場（多目的ホール・大会議室101＋102）P1会場（多目的ホール）・P2会場（会議室101＋102）
- 2P-28
金属薄膜表面支援レーザー脱離イオン化質量分析イメージング法による副腎における誘導体化ステロイドホルモンの局在解析
(1富山県立大院工・ 2金沢大国基)
o髙田莉子1・ 唐島成宙2・ 大坂一生1
Steroid hormones derived from cholesterol of a precursor are biosynthesized in the ovaries, gonads, and adrenal cortex. The localization of steroids in biological tissues are required for the assessment of physiological changes associated with disease risk and the diagnostic of various diseases simultaneously by matrix assisted laser desorption (MALDI) mass spectrometry imaging. However, it is difficult to detect due to the fact that the molecules are not easily protonated/deprotonated in MALDI. Chemical derivatization method has been used as a strategy for improving the low sensitive detection of poorly ionizable molecules by MALDI. In this study, we detect derivatized steroids in animal adrenal glands using surface-assisted laser desorption/ionization (SALDI) mass spectrometry imaging with metal film.