演題概要

2P-08

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MRMモードでのターゲット指向DESI-MSIは、マウスの腎臓におけるクロロキンの迅速な定量的MSIのための高い感度と特異性を実現します

(¹HUSM・ ²IMC, HUSM・ ³SMA・ ⁴Preppers Co. Ltd.・ ⁵NU・ ⁶Waters)
^oRahman, Md. Muedur^1,4・ Afroz, Mstsayela¹・ Mamun, Md. Al^1,4・ 坂本匠^1,4・ Islam, Ariful^4,5・ Paxton, Thanai⁶・ 佐藤智仁¹・ 華表友暁^1,2・ 髙橋豊^1,4・ 瀬藤光利^1,2,3

Mass spectrometry imaging (MSI) is a powerful technique to visualize the spatial distribution of biomolecules and drugs. Compared to MALDI-MSI and SIMS, desorption electrospray ionization (DESI) has the advantage of being a soft and ambient ionization technique ideally suited to quantitative-MSI (Q-MSI). However, there are some challenging issues related to extraction efficiency, matrix effect, sensitivity, and specificity. In this study, targeted DESI-MSI using the multiple reaction monitoring (MRM) mode was applied to address the specificity and sensitivity issues. A tandem quadrupole mass spectrometer (Xevo TQ-XS, Waters, USA) coupled with a DESI source (DESI^TM XS, Waters, USA) was used to quantify the chloroquine (m/z 320.2→247.1) as a model drug in mouse kidney tissue. The calibration curve based on a mimetic tissue model was created to quantify the distribution of the drug. The detection of chloroquine using higher sensitivity, specificity, and scan rate is demonstrated in mouse kidneys after 2 hours of drug injection at a dose of 16.7 mg/kg body weight. This study provides some valuable insights into using DESI-MSI in MRM mode for the Q-MSI study of drugs and metabolites.