演題概要

2E-L-1200

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１細胞質量分析法による細胞内代謝物局在解析

(名城大薬)
^o水野初

There are increasing demands to study the molecular and cellular behavior of a single cell precisely because the current knowledge in life science was obtained using multiple numbers of cells with few information on the localization or environment of cells. We developed the “Live Single-cell Mass Spectrometry” technique which allows direct analysis of xenobiotics and endogenous compounds in a living cell. The technique is comprised of two steps; 1. capturing of cellular content from a living cell using a metal-coated glass microcapillary (i.d. 3 μm) under microscopic observation, and 2. the sampled cellular contents such as cytosol or organelle in the capillary are directly subjected to nanoelectrospray ionization and analyzed by a mass spectrometry. We are currently trying to sample more accurate intracellular organelles using an automatic sampling device (SS2000, Yokogawa Electric Corporation). The system is to be capable of sampling a targeted whole living cell or organelle in a cell automatically based on a captured 3D confocal bioimage. We will report analytical findings of this automated live single-cell mass spectrometry, and improvement in data quality and quantitative performance.