演題概要

2A-AW-1550

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質量分析計を駆使した超高感度リゾリン脂質分析法の開発

(帝京大薬)
^o三枝大輔

Lysophosphatidic acid (LPA) and sphingosine 1-phosphate (S1P) are one of the representative lysophospholipids and related with the immune disease expression and tumor progression as the lipid mediators. However, there has been the bottleneck to detect from the biological sample due to the low abundance, and the structural isomers. Therefore, we first developed the ultra-sensitive and highly separated determination method for LPA and S1P by LC-MS/MS. We next focused the distribution of these mediators on tissue section, and showed the utilization of the laser microdissection (LMD) conduct to LC-MS/MS to demonstrate the localization of them on the brain and spleen. Although the recent technological development of MS imaging enabled us to visualize the small molecules, LPA and S1P was difficult to detect due to the in-source decay and artifact. We then applied the phos-tag technique to shift the mass spectrum from the original range for avoiding those affects, and developed the conductive adhesive film for obtaining high quality of cryosection and visualized image.