ポスター発表
第1日 6月10日（月） P会場（多目的ホール・大会議室101＋102）P1会場（多目的ホール）・P2会場（会議室101＋102）
- 1P-37
大腸菌細胞中のポリプレニル二リン酸の定量化
(1東邦大院理・ 2和歌山県立医大)
實川智貴1・ 渡邊総一郎1・ o茂里康2・ 藤崎真吾1
Although dephosphorylation of undecaprenyl diphosphate is considered important in regulating the biosynthesis of undecaprenyl phosphate, the levels of these substances have not been measured separately. We have developed a method for the measurement of the intracellular levels of these substances. Polyprenyl phosphates and diphosphates prepared by chemical phosphorylation of polyprenols from Staphylococcus aureus were used to establish the conditions for fractionation by ion-exchange chromatography and high-performance liquid chromatography (HPLC). By using an elution solvent containing tetraethylammonium phosphate as an ion-pair reagent for HPLC, polyprenyl phosphate and polyprenyl diphosphate with carbon numbers from 40 to 55 could be detected as separate peaks from the reversed-phase column. This analytical method was applied to lipids extracted from Escherichia coli to determine the intracellular levels of octaprenyl phosphate, undecaprenyl phosphate, octaprenyl diphosphate, and undecaprenyl diphosphate. This is the first report of separate measurement of cellular levels of polyprenyl phosphates and polyprenyl diphosphates.