ポスター発表
第1日 6月10日（月） P会場（多目的ホール・大会議室101＋102）P1会場（多目的ホール）・P2会場（会議室101＋102）
- 1P-24（1D-O1-1415）
UHPLC-MS/MSによるEVsターゲットリピドミクス解析手法の開発
(1帝京大薬・ 2ベーリンガー・ 3帝京医溝口病院・ 4帝京大医・ 5東北大院医・ 6東北大メガバンク)
o岩崎裕子1・ 福内友子1・ 山岡法子1・ 富樫亮2・ 松谷哲行3・ 関順彦4・ 新妻邦泰5・ 三枝大輔1,6
It has been suggested that proteins, nucleic acids and lipid species contained in extracellular vesicles (EVs) differ depending on the onset and progression of disease. However, current lipidomics analysis is insufficiently precise, and the establishment of an accurate quantitative analysis method is required. We therefore developed an EVs targeted lipidomics method using UHPLC-MS/MS. Then, EVs targeted lipidmics analysis by UHPLC-MS/MS successfully detected lipid molecular species including ceramides and phospholipids, lysophospholipids, ceramides, hexosylceramides, cholesterylesthers, and triglyceride. Our targeted lipidmics may utilize to the biomarker discovery for plasma derived EVs and demonstrate EVs’ related disease mechanism.