ポスター発表
第1日 6月10日（月） P会場（多目的ホール・大会議室101＋102）P1会場（多目的ホール）・P2会場（会議室101＋102）
- 1P-23
マクロファージ様細胞株の13C代謝フラックス解析法の構築
(阪大院情報)
o橋本隼輔
13C-metabolic flux analysis using mass spectrometry was developed to investigate a metabolic alteration in immune cell differentiations. 13C-tracing analysis of THP-1, a human leukemia cell line, revealed uptakes of extracellular glutamate and aspartate as well as degradations of intracellular macromolecules. By incorporating these reactions into a metabolic model, flux distributions were calculated. The results showed that differentiation into macrophages dramatically increase the glycolytic flux, while the TCA cycle flux were moderately increased.