演題概要

1P-04

PDF

ネイティブ質量分析におけるnanoESIによって生成されるイオンの温度計イオンによる評価

(¹横市大院生命医・ ²産総研)
^o田尻道子¹・ 七種和美²・ 池上貴久¹・ 浅川大樹²・ 明石知子¹

Native mass spectrometry (native MS) is a method that uses neutral volatile solvents to ionize protein complexes formed by weak non-covalent interactions, which can then be analyzed by mass spectrometry. Smaller sprayed droplet sizes are considered to result in softer ionization, and nanoelectrospray ionization (nanoESI) is used for native MS. In this study, nanoESI MS was performed under both denaturing and native conditions utilizing benzylammoniums, recently identified as suitable thermometer ions for estimating the internal energy distribution of ions produced by ESI. By comparing the internal energy distributions observed under these two conditions, ions produced by native MS were evaluated and discussed. Furthermore, the internal energy distribution of the ions, which varies with droplet size under native conditions, was quantitatively investigated.