基盤セッション
第1日 6月10日（月） 14:51～15:09 C会場（中会議室201）
- 1C-O1-1451（1P-33）
5,000試料連続測定における瞬速マシンガンプロテオミクスの性能評価
(1京大院薬・ 2医薬基盤健栄研・ 3理研IMS)
o富岡郁那1・ 富岡亮太1・ 金尾英佑1,2・ 小形公亮1・ 今見考志3・ 杉山直幸1・ 石濱泰1,2
Bottom-up proteomics using nanoLC/MS/MS has become widely used in clinical applications and in the elucidation of biological function, but balancing its throughput with robustness, sensitivity, and analytical depth remains a challenge. Increasing flow rate and shortening gradient time have been examined to improve throughput, but often result in lower sensitivity and lower depth of analysis than standard nanoLC/MS/MS. Here, we focused on capillary-flow LC to maintain sensitivity and developed an ultrahigh-speed LC/MS/MS that achieves 1.4 minutes per run, equivalent to 1000 samples per day. The method was shown to be remarkably robust, capable of more than 5,000 continuous measurements per week, including maintenance time.