演題概要

1C-O1-1415（1P-32）

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電子誘起解離を用いたタンデム質量分析によるN末端プロテオミクス

(¹京大院薬・ ²AB SCIEX・ ³医薬基盤健栄研)
^o小形公亮¹・ 谷口優斗¹・ 柴田猛²・ 石濱泰^1,3

In this study, we evaluated peptide fragmentation using electron activated dissociation (EAD) to enhance the identification efficiency of peptides lacking basic functional groups. These peptides can be generated from protein N-terminus through LysargiNase digestion. Through the analysis, we identified 1124 N-terminal peptides using conventional collision induced dissociation (CID) and 1084 N-terminal peptides using EAD. There were 680 peptides that overlapped between the two datasets. By comparing the identification scores of 627 peptide ions with the same peptide sequence and precursor charge, we found that the average score was 23.6 for CID and 73.5 for EAD. Notably, for all peptides lacking basic functional groups, the scores obtained through EAD were higher than those obtained through CID, demonstrating the superiority of EAD.