若手研究者セッション
第1日 6月10日（月） 15:00～15:15 B会場（中ホール200）
- 1B-O1-1500（1P-29）
構造プロテオミクスにおける高深化限定消化法の開発
(1京大院薬・ 2医薬基盤健栄研)
o村松邑香1・ 小形公亮1・ 石濱泰1,2
Protein higher-order structure is closely related to its molecular function. Proteome-scale structural analysis, or structural proteomics, is essential to elucidate the effects of external environmental stimuli on protein conformational changes. Limited Proteolysis-coupled Mass Spectrometry (LiP-MS) 1), which combines two-step protease digestion and mass spectrometry, has been developed to realize structural proteome analysis. However, the protocol is not yet well matured, and experimental conditions to effectively search for protein conformational changes on a proteome scale need to be further investigated. In this study, we examined various experimental conditions including partial digestion, to comprehensively detect structural changes involved in the protein function.