演題概要

1A-S-1710

PDF

Shotgun Proteomic Analysis of Akkermansia Muciniphila-Secretome for Discovery of Pharmabiotics from Human Gut Microbiome

(¹KRISS・ ²KRIBB)
Kim, Young Eun¹・ Kang, Eun-Jung²・ Kim, Jae-Hoon²・ Kim, Yong-Hoon²・ Lee, Chul-Ho²・ ^oKang, Dukjin¹

Very recently, the remarkable impact of gut microbiota has been gaining increasing attention as a key role to maintain host health by regulating host metabolism and immunity. However, the biological functions of the gut microbiome engaging in intricate symbiotic interactions are unclear. Importantly, emerging research suggests that the interplay between the gut microbiome and the intestines can influence the onset of conditions such as inflammatory bowel disease (IBD), heart diseases, and both type 1 and type 2 diabetes. In this presentation, we introduce a cutting-edge automated shotgun proteomics approach designed to identify Akkermansia muciniphila (AK)-cellular /-secreted proteome via 2D-nLC-ESI-MS/MS with isobaric labeling and label-free quantification approaches to unearth pharmabiotics candidates from AK. From quantitative profiling of AK-secreted proteome prepared at varied culture conditions, we have uncovered a novel protein, Amuc_1409, which holds promise for offering health-promoting effects on human gut health whereby this Amuc_1409 promoted E-cadherin/β-catenin complex dissociation via interaction with E-cadherin, resulting in the activation of Wnt/β-catenin signalling. Our work underpins the exciting potential of proteomics in advancing our understanding of the intricate relationships between gut microbes and human health.