シンポジウムセッション
第1日 6月10日（月） 16:55～17:10 A会場（中ホール300）
- 1A-S-1655
１細胞質量分析法による細胞内リン脂質分析法の開発
(1名城大薬・ 2静岡県大薬・ 3伊藤園)
o水野初1,2・ 古庄仰2・ 阪田晟2・ 栁澤拓摩1,2・ 杉山栄二2・ 今西進1・ 轟木堅一郎2・ 坂根巌3
In this study, we have developed a method for selective and sensitive analysis of phospholipids constituting cell membranes at the single-cell level. In addition, optimal detergents were investigated to solubilize cell membranes and extract phospholipids. Phospholipids are assumed to play vital roles in cell membrane composition and in realizing signal transduction. Phospholipid profiles vary distinctively with cell types; particularly, specific phospholipid molecules exhibit notable increases or decreases in cancer cells. This method was applied to phospholipid analysis of cancer and normal blood cell models, and each cell-specific phosphatidylcholine peak was detected.