演題概要

オーラルセッション

第３日　５月２０日（金）　9:15～9:30　C会場（星雲２）

3C-O1-0915（3P-34）　　PDF

高分解能タンデム飛行時間型質量分析装置(TOF/TOF)でのジスルフィド含有プロトン化ペプチドの高エネルギー電子移動解離(HE-ETD)

(¹阪府大院理・ ²阪大院理・ ³産総研)
^o曽木政伸¹・ 藤原亮正¹・ 豊田岐聡²・ 今岡成章²・ 茂里康³・ 早川滋雄¹

Disulfide linkages in polypeptides are post-translational modifications that play a pivotal role in maintaining higher order structure and biological functions. In this work, high-energy electron transfer dissociation (HE-ETD) using Cs targets was applied to doubly protonated disulfide linked polypeptides, such as vasopressin and vasotocin. From both cleavage of peptide bond and S-S bond, it was found that the peptide sequence having S-S bond was able to be completely determined by HE-ETD spectra. C-S bond of the cysteine residue in the central part cleaved easier than that in the N-terminal.