演題概要

オーラルセッション

第１日　５月１８日（水）　16:45～17:05　A会場（オービットホール）

1A-O1-1645　　PDF

質量スペクトルはデータベース検索“グレーゾーン”を明瞭化するか

(¹京大化研・ ²京大院薬・ ³DBCLS・ ⁴新潟大院医歯・ ⁵新潟大産学・ ⁶九大生医研・ ⁷熊本大院生命)
^o吉沢明康¹・ 田畑剛²・ 守屋勇樹³・ 河野信³・ 奥田修二郎⁴・ 山本格⁵・ 松本雅記⁶・ 小林大樹⁷・ 荒木令江⁷・ 杉山直幸²・ 五斗進¹・ 石濱泰²

The database search protocol is the mainstream for current proteomics analyses. However, the results by this protocol usually contain false positive hits, requiring careful interpretations of the results. Based on this fact, we are now developing a methodology to remove these "results in the twilight zone," namely unreliable results that can be false positives, from the search results, in order to develop an integrated proteome database "jPOST," which has been developed since last year. For this methodology, we are focusing the features of obtained mass spectra to select reliable spectra equivalent to manual inspection. In this presentation, we will introduce the development and the analyses for jPOST and discuss the improvement of database search results by the careful analyses of mass spectra.