Oral Sessions (Day1, Day2, Day3)
Poster Presentations
(Day1, Day2, Day3)
Poster Presentations
- Day 3, May 17（Fri.） Room P (Multi-purpose Hall)
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3P-17 PDF
Development of Sample Preparation Method to Improve Sensitivity and Reproducibility of Mass Spectrometry-Based Metabolite Imaging
Matrix-assisted laser desorption ionization (MALDI)-mass spectrometry imaging (MSI) enables to visualize the distribution of biomolecules. The choice of the optimized matrix deposition method is critical for visualizing distribution of biomolecules with high sensitivity and high spatial resolution. Generally, manual or automated spray-coating and automated sublimation methods are used, but these methods have some drawbacks with respect to detection sensitivity, spatial resolution, and data reproducibility. Herein, we present the 9-aminoacridine (9-AA) deposition method of sublimation coupled with recrystallization. The matrix recrystallization process after sublimation was optimized for the solvent concentration and reaction temperature. After sublimation, a 9-AA-coated glass slide was exposed to 5-20% methanol vapor at 30-80°C in a reaction chamber constructed in our laboratory. We found that 5% methanol at 60°C condition can provide clear images of the representative 9-AA-sensitive nucleotide derivatives. Next, we investigated the three different matrix application methods (automated spray-coating, sublimation, and sublimation coupled with recrystallization methods) for their detection sensitivity, spatial resolution and reproducibility. Our optimized method showed the best detection sensitivity and provided clear images for nucleotide derivatives and lipids. In addition, this method provided higher reproducible MSI data with higher spatial resolution than the other two methods.