Abstract

Poster Presentations

Day 1, May 15（Wed.）　　Room P (Multi-purpose Hall)

1P-17　　PDF

Development of a Practical Online SFE-SFC/MS System with a Novel Split-Flow Method

(¹Miyazaki Agri. Res. Inst., ²Shimadzu, ³Food Res., ⁴Osaka Univ., ⁵Kyushu Univ.)
Miho Sakai¹, Yoshihiro Hayakawa², Yasuhiro Funada², Takashi Ando³, Eiichro Fukusaki⁴, ^oTakeshi Bamba⁵

In this study, we developed a practical online supercritical fluid extraction-supercritical fluid chromatography/mass spectrometry (SFE-SFC/MS) system with a novel split-flow method, which enabled the continuous extraction and separation of a wide range of compounds including hydrophilic ones. In this system, a part of the extract was introduced into the separation section due to the differential pressure of two BPRs and make-up pump flow rate to reduce the peak distortion of target analyte caused by an excess of extract-containing extractant. Moreover, the separation was improved by the use of an additional pre-column capable of interacting with compounds weakly retained on the analytical column.