オーラルセッション
- 第3日 5月17日（金） 10:10～10:30 C会場（101）
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3C-O1-1010 PDF
HTSや初期動態研究における質量分析プレートリーダーの可能性
Label-free LC/MS based screening technology is routinely used in pharmaceutical industries for hit discovery and various ADME profiling applications. Although the current analysis speed of <30 seconds per sample is quite promising, it still cannot match the throughput provided by plate-reader based HTS platforms. Acoustic droplet ejection (ADE) is a droplet transfer technology capable of high speed, reproducibility and absolute accuracy. In this work, we successfully couple ADE and the standard ESI ion source of a mass spectrometer with the open-port probe (OPP) sampling interface. Screening speeds as fast as 0.4 seconds-per-sample are demonstrated with superb sensitivity, great reproducibility even without internal standard, good quantitation capability, no matrix effect, and broad compound coverage.