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オーラルセッション

第3日　5月17日（金）　16:05～16:25　B会場（102）

3B-O2-1605　　PDF

MALDI - イメージング質量分析法を用いた多発性硬化症のプロテオミクスレベルのバイオマーカーと治療標的の特定

(¹同志社大生命医・ ²ブルカージャパン・ ³京府医大・ ⁴関西医大)
^o田中那美¹・ 山下浩輝¹・ 韮澤崇²・ 梶田遼²・ 田口勝敏³・ 田中雅樹³・ 近藤誉之⁴・ 辻雄大¹・ 角田伸人¹・ 池川雅哉¹

Multiple sclerosis (MS) is an autoimmune disease; its cardinal symptoms are chronic inflammatory, demyelination and axonal degeneration of central neuro system (CNS). In this study, we applied matrix assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) for　CNS of murine model of MS; experimental autoimmune encephalomyelitis (EAE). We have successfully identified and visualized several proteins specifically expressed in lesions of EAE at preclinical to clinical stages. It was functionally validated on an EAE model with or without blocking agents of the proteins. Furthermore, in depth proteomics using LC-TIMS-MS/MS for the cells infiltrated into CNS was also performed. In understanding molecular mechanisms of disease progression as well as pharmacological effects of the agents above mentioned, we have utilized whole animal IMS at the proteomics level.