日本質量分析学会 第67回質量分析総合討論会

演題概要

オーラルセッション

第3日 5月17日(金) 10:10~10:30 B会場(102)

心疾患及び腎疾患患者血清の微量元素分析

(1東レリサーチセ2宇治病院)
o一ノ瀬尊之1坂口晃一1佐藤信之1平岡勇二2

Many trace elements are claimed to relate with many diseases. However, it is also known that there are some difficulties in the analysis of trace elements in biological resources, for example, spectral interferences, sensitivity, and loss of volatile elements. We have established the analytical method for the measurements of trace elements in serum using inductively coupled plasma – sector field mass spectrometry (ICP-SFMS) and microwave digestion system.
For the preparation of sample solutions, we used closed vessel – microwave digestion system to avoid the loss of volatile elements. Nineteen elements (Li, B, Al, Ti, V, Cr, Mn, Co, Ni, As, Se, Sr, Mo, Cd, Sn, Sb, Ba, Pt, and Pb) were measured by ICP-SFMS to avoid spectral interferences and to improve sensitivity, and 6 elements (Ca, Mg, P, Fe, Cu, and Zn) were also monitored by ICP-atomic emission spectrometry (ICP-AES).
Twenty five elements in human serum of the patients with heart or kidney disease and healthy controls were analyzed. From the result of multiple regression analysis, we examine the relationship between trace elements and those diseases. The results showed that Se and Mn concentration decreased in the patients with heart disease significantly, meaning that Se and Mn relates with antioxidant enzymes, such as GPx and SOD. On the other hand, Cr, Mo, and Ni concentration increased in the patients with kidney disease on artificial dialysis.