The 72nd Annual Conference on Mass Spectrometry, Japan
Date:
Mon, Jun 10, - Wed, Jun 12, 2024
Venue:
Tsukuba International Congress Center (Takezono, Tsukuba City, Ibaraki Prefecture 305-0032, Japan)
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Abstract

Poster Presentations

Day 3, June 12(Wed.)  Room P1 (Multipurpose Hall)・Room P2 (Conference Room 101+102)

3P-43
PDF

Distribution Analysis of Related Substances to Elucidate the Function of Phytoalexins in Plant-Pathogen Interactions

(1Shimadzu, 2Ryukoku Univ.)
Kaoru Nakagawa1, Kouji Okuda1, Eriko Betsuyaku2, oQiuyi Wang1, Manami Kobayashi1, Shigeyuki Betsuyaku2

Phytoalexins are secondary antimicrobial compounds that are rapidly accumulated de novo upon pathogen attacks. However, compared to their well-known functions against fungi and oomycetes, their roles in plant-bacterial interactions remained obscure as exemplified by camalexin, the major phytoalexin in Arabidopsis. Our previous study revealed that the promoter activity of PHYTOALEXIN DEFICIENT 3 (PAD3), a gene required for the camalexin synthesis, is specifically suppressed in the leaf area infiltrated with Pseudomonas syringae pv. tomato DC3000 (Pst) in a Type III Secretion System-dependent manner. We will present our further dissection of this bacterial suppression of camalexin (CA), including mass spectrometry imaging techniques using the iMScopeTM QT (Shimadzu Co.) and the LA -ICPMS-2030 (Shimadzu Co.).