Abstract

Poster Presentations

Day 3, June 12（Wed.）　　Room P1 (Multipurpose Hall)・Room P2 (Conference Room 101+102)

Tap water disinfection by chlorine plays a key role to ensure the availability of potable water. However, residual chlorine can be depleted by tap water stagnation, and bacterial regrowth occurs. In terms of drinking water safety, it is of importance to establish a method to control unwanted bacterial regrowth. Although reducing biodegradable organic matters (BOMs) is a fundamental approach to control the regrowth, BOM is not well characterized. In this study, a non-targeted analysis using liquid chromatography/high resolution mass spectrometry was adopted to explore and identify BOMs in tap water.
Fresh tap water samples were collected at the University of Tokyo and they were incubated for five days. After the incubation, total cell count of the samples were increased by more than 300 times, showing significant bacterial regrowth. The samples were concentrated using solid phase extraction and the obtained eluate underwent non-targeted LC/MS analysis, which resulted in the detection of 12,628 components. Ten possible BOM candidates were obtained through the screening these components, and two of them were successfully identified as N-lauroylglycine and N-myristoylglycine using their authentic standards. More detailed study is now in progress to validate their bioavailability.

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