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Poster Presentations
Day 3, June 12（Wed.） Room P1 (Multipurpose Hall)・Room P2 (Conference Room 101+102)
- 3P-24
Comprehensive Analysis of Free Amino Acids and Short-Chain Fatty Acids in Human Serum by Gas Chromatography Mass Spectrometry
(1Toyama Pref. Univ., 2Kanazawa Univ.)
oNozomi Nakamura1, Shigehiro Karashima2, Issey Osaka1
Relationship between the genome and the phenotype in organisms have been studied. Understanding the state of the genes and proteins does not reveal it. Metabolomics has become an important in clinical research and the diagnosis for human disease. The metabolomics for identifying biomarkers screening biomarkers of diseases has been studied using mass spectrometry. Generally, analysis of metabolites is performed by liquid chromatography-mass spectrometry (LC/MS). In metabolomic analysis for Gas chromatography-mass spectrometry (GC/MS), many polar metabolites require derivatization. In this study, we investigated comprehensive analytical method using GC/MS for metabolomics. EI equipped to GCMS is useful to produce many potential fragment ions for the structural analysis. Free amino acids and short-chain fatty acids and other metabolites in human serum were detected.